We study biomolecular reactions that convert electrochemical energy into chemical bonds of reduced products. This research advances the development of enzyme-based and microbial-based systems for the production of energy compounds and carriers.
[FeFe]- and [NiFe]-Hydrogenase Diversity, Mechanism, and Maturation, Biochimica et Biophysica Acta - Molecular Cell Research (2015)
[FeFe]-Hydrogenase Oxygen Inactivation Is Initiated by the Modification and Degradation of the H Cluster 2Fe Subcluster, Journal of the American Chemical Society (2015)
View all NREL physical biochemistry publications.
To evaluate the paramagnetic properties of redox active cofactors in metallo- and flavoproteins we have a spin resonance facility that houses a Bruker E-500 EPR and a E-580 pulse EPR with closed-cycle, He-cryostats (temperature range >4K), and a fiber optic laser and optical electron paramagnetic resonance (EPR) probes.
Our capabilities include a range of infrared, mid-infrared, and ultrafast transient absorption (UV/visible) spectrometers that facilitate the study of very fast electron transfer events in a variety of redox active cofactors.
We have developed enzyme-nanoparticle complexes as photochemical systems to couple light harvesting to drive electron conversion reactions for deciphering fundamental mechanisms of electron transfer and catalysis in redox enzymes.
We have developed recombinant expression systems for the production of a range of different metalloproteins including hydrogenases, hydrogenase maturases, ferredoxins, and flavin-containing enzymes.
Rachel Ward (University of Colorado Boulder)
Arizona State University, Jones Research Group
Carnegie Mellon University, Guo Research Group
Montana State University, Bothner Lab
Montana State University, Broderick Research Group
University of Colorado Boulder, Dukovic Research Group
University of Colorado Boulder, Smalyukh Research Group
University of Georgia Athens, Adams Research Group
University of Kentucky, Miller Research Group
Utah State University, Seefeldt Research Group
Funding for NREL's Redox Biochemistry R&D is provided by the U.S. Department of Energy, Office of Science, Basic Energy Sciences Program, and the Biological and Electron Transfer and Catalysis EFRC, an Energy Frontiers Research Center funded by the U.S. Department of Energy, Office of Science.