Bio-Oil Analysis Laboratory Procedures

NREL develops laboratory analytical procedures (LAPs) for the analysis of raw and upgraded pyrolysis bio-oils. These standard procedures have been validated and allow for reliable bio-oil analysis.

Procedures

Determination of Carbon Functional Groups in Pyrolysis Bio-Oils Using 13C NMR

This procedure quantifies different carbons in the different functional groups in pyrolysis bio-oils, including carboxyl groups; aromatic C-O, C-C, and C-H bonds; six different carbons in levoglucosan; aliphatic C-O and C-C bonds; methoxyl groups; and two different methyl groups.

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Determination of Water Content in Bio-Oils by Volumetric Karl Fischer Titration

This method analyzes the concentration of water in fast pyrolysis oil, catalytic fast pyrolysis oil, and aqueous phases isolated from these products. Other products can be measured using this technique if water concentration is within the approximate range of 0.1 to 100 wt%.

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Determination of Carbon, Hydrogen, Nitrogen, and Oxygen in Bio-Oils

This procedure uses combustion analysis to determine the weight percent of carbon, hydrogen, and nitrogen in fast pyrolysis oil (FP), catalytic fast pyrolysis oil (CFP), distillate range hydrotreated oil products, and aqueous phases isolated from FP and CFP oils.

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Accelerated Aging of Fast Pyrolysis Bio-Oil Using Carbonyl Titration

Bio-oils change both physically and chemically during storage. This procedure accelerates the aging of fast pyrolysis bio-oils and can be used to gauge how a sample has changed, or will change, with varying times of storage at room temperature.

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Determination of Carbonyls in Pyrolysis Bio-Oils by Potentiometric Titration: Faix Method

This procedure uses potentiometric titration to quantify carbonyl functional groups in raw pyrolysis bio-oils. Carbonyl groups are characterized as the sum of aldehydes and ketones.

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Acid Number Determination of Pyrolysis Bio-Oils Using Potentiometric Titration

This procedure measures the acidic content of bio-oils using potentiometric titration. Acid content is characterized through both the carboxylic acid number (CAN) and the total acid number (TAN).

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Determination of Hydroxyl Groups in Pyrolysis Bio-Oils Using 31P NMR

This procedure uses phosphorus-31 nuclear magnetic resonance (31P NMR) to quantify three different hydroxyl groups (-OH) in pyrolysis bio-oil: aliphatic-OH, phenolic-OH, and carboxylic-OH.

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Quantification of Semi-Volatile Oxygenated Components of Pyrolysis Bio-Oil by Gas Chromatography/Mass Spectrometry (GC/MS)

This procedure quantifies semi-volatile oxygenated compounds present in raw bio-oils produced from fast pyrolysis of lignocellulosic feedstocks.

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Contact

For more information about these laboratory analytical procedures, contact Jack Ferrell, 303-384-7777.