Skip to main content

Research Interests

  • Development of genetic tools for analysis and metabolic engineering of diverse microbes

  • CO and CO2 metabolism

  • Hydrogenases and H2 metabolism

  • Engineering novel microbial platforms for biofuels/bioproducts

  • Development of high throughput screening and selection methods


Affiliated Research Programs

  • Conversion of Syngas to Terpenes in a Thermophilic Biocatalyst, U.S. Department of Energy (DOE) Bioenergy Technologies Office (BETO) Incubator, co-PI, PI: Kiverdi, Inc., Berkeley, California

  • Center for Bioenergy Innovation (CBI), Rapid Genetics Team lead and co-PI, DOE Biological and Environmental Research (BER) Bioenergy Research Center, PI: Jerry Tuskan (Oak Ridge National Laboratory)

  • Design and Engineering of Synthetic Control Architectures, co-PI, DOE BER Biosystems Design, PI: Ryan Gill (University of Colorado, Boulder)

  • Progress towards a new model chemolithoautotrophic host, BETO Agile BioFoundry, co-PI, Lawrence Berkeley National Laboratory lead, with Kiverdi, Inc.


Areas of Expertise

  • Genetic and metabolic engineering and analysis of diverse microbes

    • Saccharomyces cerevisiae

    • Escherichia coli

    • Cyanobacteria (Synechocystis, Synechococcus)

    • Cupriavidus necator (Ralstonia eutropha H16)

    • Rubrivivax gelatinosus CBS

    • Caldanaerobacter subterraneus subspecies tengcongenesis

    • Clostridium thermocellum

    • Pseudomonas putida KT2440

    • Zymomonas mobilis

  • Genetic tool development (transformation tools, gene knockout/expression, synthetic biology, Clustered Regularly Interspaced Short Palindromic Repeats [CRISPR])

  • High-throughput genome editing technologies

  • Hydrogenases and H2 metabolism

  • CO2 fixation

  • Photosynthesis

  • CO metabolism


Education

  • Ph.D., Molecular Biology, University of Colorado, Anschutz Campus, 2001–2006

  • B.S., Biology, University of South Dakota, 19951999


Professional Experience

  • Director, Center for Biodesign Works—Renewable and Sustainable Energy Institute, University of Colorado, Boulder, 2016present

  • Senior Research Associate—Renewable and Sustainable Energy Institute, University of Colorado, Boulder, 2013–present

  • Senior Scientist—genetic engineering of diverse microbes, National Renewable Energy Laboratory (NREL), Biosciences Center, 2011present

  • Postdoctoral Fellow—Photobiology group (Pin-Ching Maness, PI), NREL, Biosciences Center, 20082011

  • Postdoctoral Fellow—Cell Cycle in Xenopus laevis (James Maller, PI), Howard Hughes Medical Institute, University of Colorado, Anschutz Campus, 20062008

  • Doctoral candidate—Chromosome segregation in Saccharomyces cerevisiae (Paul Megee, PI), University of Colorado, Anschutz Campus, 20012006


Featured Publications

  1. "A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803," ACS Synthetic Biology (2018)

  2. "Overcoming substrate limitations for improved production of ethylene in E. coli.," Biotechnology for Biofuels (2016)

  3. "Genome Annotation Provides Insight into Carbon Monoxide and Hydrogen Metabolism in Rubrivivax gelatinosus," PLOS One (2014)

  4. "Solar powered biohydrogen production requires specific localization of the hydrogenase," Energy and Environmental Science (2014)

  5. "Ethylene forming enzyme and bio-ethylene production," Biotechnology for Biofuels (2014)

  6. "Hydrogen production by water biophotolysis," in Microbial BioEnergy (2014)

  7. "Genetic analysis of the Hox hydrogenase in the cyanobacterium synechocystis sp. PCC 6803 reveals subunit roles in association, assembly, maturation, and function," Journal of Biological Chemistry (2012)

  8. "Hydrogenase Genes and Enzymes Involved in Solar Hydrogen Production," in State of the Art and Progress in Production of Biohydrogen (2012)

  9. "The role of the bidirectional hydrogenase in cyanobacteria," Bioresource Technology (2011)

  10. "Recombinant and In Vitro Expression Systems for Hydrogenases: New Frontiers in Basic and Applied Studies for Biological and Synthesis H2 Production," Dalton Transactions (2012)

  11. Photobiological Hydrogen ProductionProspects and Challenges, Microbe (2009)

  12. "The enhancement of pericentromeric cohesin association by conserved kinetochore components promotes high-fidelity chromosome segregation and is sensitive to microtubule-based tension," Genes Dev (2007)

View all NREL Publications for Carrie Eckert.


Please contact me with research questions and ideas for collaborations. Please do NOT contact me directly for jobs—see instead information on NREL's Director's Postdoctoral Fellowship program or on NREL Careers in general.