Vladimir Lunin

Vladimir Lunin

Senior Scientist

Protein Crystallography

Photo of Vladimir Lunin
(303) 384-7754
At NREL Since: 

Vladimir Lunin is an experienced protein crystallographer. His primary research interests are structure-functional studies of polysaccharide-degrading enzymes with a focus on glycoside hydrolase family 7.
Areas of Expertise

• Protein purification
• Protein crystallization
• X-ray diffraction data collection
• Protein structure determination
• Obtaining structures of protein-ligand complexes
• Site-directed mutagenesis
• Structure-function relationship
• Enzymatic mechanisms

Research Interests

• Glycoside hydrolases, lyases, transferases
• Establishing molecular mechanisms of protein-substrate interaction for enzymes involved in polysaccharide synthesis, modification or breakdown
• Biomass deconstruction and recalcitrance


  • 2008 National Renewable Energy Laboratory

  • 2004–2007 University Health Network, Toronto, Canada

  • 2001–2004 Biotechnology Research Institute, National Research Council, Montreal, Canada

  • 2000 Ph.D. in Chemistry from Institute of Crystallography, Russian Academy of Sciences

  • 1997 M.S. in Chemistry, Moscow State University

Selected Publications 

  1. Brunecky, Roman ; Alahuhta, Markus; Xu, Qi; Donohoe, Bryon; Crowley, Michael F. ; Kataeva, Irina A.; Yang, Sung-Jae; Resch, Michael G. ; Adams, Michael W. ; Lunin, Vladimir V. ; Himmel, Michael E. ; Bomble, Yannick J. (2013). "Revealing Nature's Cellulase Diversity: The Digestion Mechanism of Caldicellulosiruptor bescii CelA." Science (342); pp. 1513-1516.
  2. Leonid O Sukharnikov; Markus Alahuhta; Roman Brunecky; Amit Upadhyay; Michael E Himmel; Vladimir V Lunin*; Igor B Zhulin* (2012). "Sequence, Structure, and Evolution of Cellulases in Glycoside Hydrolase Family 48." Journal of Biological Chemistry pp. 41068-41077.
  3. Alahuhta, M.; Xu, Q.; Bomble, Y.J.; Brunecky, R.; Adney, W.S.; Ding, S-Y.; Himmel, M.E.; Lunin, V.V. (2010). "The Unique Binding Mode of Cellulosomal CBM4 from Clostridium thermocellum Cellobiohydrolase." A. J. Mol. Biol. (402); pp. 374-387.
  4. Sara E Blumer-Schuette; Markus Alahuhta; Jonathan M Conway; Laura L Lee; Jeffrey V Zurawski; Richard J Giannone; Robert L Hettich; Vladimir V Lunin; Michael E Himmel; Robert M Kelly* (2015). "Discrete and Structurally Unique Proteins (Tāpirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose." Journal of Biological Chemistry pp. 10645-10656.
  5. Lunin, V.V.; Dobrovetsky, E.; Khutoreskaya, G.; Zhang, R.; Joachimiak, A.; Doyle, D.A.; Bochkarev, A.; Maguire, M.E.;; Edwards, A.M.; Koth, C.M. (April 2006). "Crystal Structure of the CorA Mg 2+ Transporter." Nature (440); pp. 833-837.