Michael Resch

Michael Resch

Research Scientist

(303) 384-7854
At NREL Since: 

Michael Resch holds a Ph.D. in Biochemistry and Molecular Biology from Colorado State University. His dissertation work focused on the biophysical and structural properties of nucleosomes and chromatin arrays in vitro in the laboratories of Dr. Karolin Luger and Dr. Jeffery Hansen at Colorado State University.

Dr. Resch's NREL career began in 2008 as a postdoctoral researcher working on enzyme characterization in the context of cellulosomes and free enzyme cellulase and hemicellulase systems. In early 2011, he was hired as a research scientist and has focused on purifying and characterizing cell-free cellulosomes and individual hemi-cellulase enzymes. He uses protein crystallography, small-angle X-ray scattering, analytical ultracentrifugation, FPLC chromatography, and poly-acrylamide gel electrophoresis to characterize the biophysical nature of these systems. He also investigates cellulase and hemicellulase enzymatic properties by analyzing the digestion of biomass and model cellulose substrates. This work will help NREL discover how these enzyme systems can improve the efficiency of converting biomass to sugars for biofuel production.

Selected Publications 

  1. Resch, M.G; Donohoe, B.S; Baker, J.O.; Decker, S.R; Bayer, E.A. ; Beckham, G.T.; Himmel, M.E. "Complementary mechanisms of plant cell wall deconstruction by free and complexed enzymes."
  2. Brunecky, R. ; Donohoe, B. S.; Selig, M.J.; Wei, H.; Resch, M.G.; Himmel, M.E. "Basic biological research relevant to feedstock conversion."
  3. Panchenk,o T.; Sorensen T.C.; Woodcock, C.L.; Kan, Z.Y.; Wood, S.; Resch M.G.; ; Luger, K.; Englander, S.W.; Hansen J.C.; Black B.E. (2011). "Replacement of histone H3 with CENP-A directs global nucleosome array condensation and loosening of nucleosome superhelical termini." Proceedings of the National Academy of Sciences of the United States of America (108:40); pp. 16588-16593.
  4. Beckham, G.T.; Bomble, Y.J.; Matthew, J.F.; Taylor, C.B.;; Resch, M.G.; Yarbrough, J.M.; Decker, S.R.; Bu, L.; Zhao, X.;; McCabe, C.; Wohlert, J.; Bergenstråhle, M.; Brady, J.W.; Adney, W.S.; Himmel, M.E.; Crowley, M.F. (December 2010). "The O-glycosylated linker from the Trichoderma reesei Family 7 cellulase is a flexible, disordered protein." Biophysical J. (99:11);
  5. Resch, M.G.; Watanabe, S.; Lilyestrom, W.; Clark, N.; Hansen, J.C.; Peterson, C.; Luger, K. (May 2010). "Structural characterization of the H3K56Q nucleosomes and nucleosomal arrays." Biochim Biophys Acta (1799(5-6):480-6);
  6. McBryant, S.J.; Klonoski, J.; Sorensen, T.C.; Norskog, S.S.; Williams, S.; Resch, M.G.;; Toombs, III, J.A.; Hobdey, S.E.; Hansen, J.C. (June 2009). "Determinants of histone H4 N-terminal domain function during nucleosomal array oligomerization: Roles of amino acid sequence, domain length, and charge density." J Biol Chem. (284:25); pp. 16716-16722.
  7. Lu, X.; Klonoski, J.M.; Resch, M.G.; Hansen, J.C. (August 2006). "In vitro chromatin self-association and its relevance to genome architecture." Biochem Cell Biol. (84:4); pp. 411-417.
  8. Brunecky, Roman ; Alahuhta, Markus; Xu, Qi; Donohoe, Bryon; Crowley, Michael F. ; Kataeva, Irina A.; Yang, Sung-Jae; Resch, Michael G. ; Adams, Michael W. ; Lunin, Vladimir V. ; Himmel, Michael E. ; Bomble, Yannick J. (2013). "Revealing Nature's Cellulase Diversity: The Digestion Mechanism of Caldicellulosiruptor bescii CelA." Science (342); pp. 1513-1516.