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Low Molecular Weight Thermostable .Beta.-D-Glucosidase from Acidothermus Cellulolyticus

U.S. Patent 5,432,075


Technology Description

A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.

Inventors

Michael E. Himmel; Rafael A. Nieves; William S. Adney; Melvin P. Tucker

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